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MiSeqDX System & MiSeq System (ILLUMINA)

The MiSeq System offers the first end-to-end sequencing solution, integrating cluster generation, amplification, sequencing, and data analysis into a single instrument.
MiSeq is the only benchtop sequencer that can produce 2 x 300 paired-end reads and up to 15 Gb of data in a single run. This allows assembly of small genomes or detection of target variants with unmatched accuracy, especially within homopolymer regions. Now, even more samples can be processed in less time while generating more reads per run than any previous versions.

The MiSeq System offers a cost-effective alternative to capillary electrophoresis (CE) for applications such as targeted resequencing, clone checking, and amplicon sequencing.

The MiSeqDx System is the first NGS platform to receive FDA clearance for in vitro diagnostic (IVD) testing.

Most Frequent Applications

Small genome sequencing, 16S/18S metagenomics, smallRNA sequencing, HLA sequencing, preimplantation genetic screening (PGS), and preimplantation genetic diagnosis (PGD), cystic fibrosis, amplicon sequencing.

Highlights

Multiplex up to 96 samples per run for greater efficiency

Get accurate bi-directional amplicon sequencing

Generate more complete de novo assemblies for small genome

Technical Specifications

MiSeq Reagent kit v3MiSeq Reagent kit v2MiSeq Reagent kit v2 MicroMiSeq Reagent kit v2 Nano
No. of Samples / TimeNo. of Samples / TimeNo. of Samples / TimeNo. of Samples / Time
Amplicon Resequencing>250X Coverage 2x150bp96 / 28 Hours96 / 24 Hours96 / 19 Hours96 / 17 Hours
16S Metagenomics 1M Reads2x250bp40 / 47 Hours20 / 39 Hours2 / 28 Hours
Small-Genome Sequencing>30X Coverage2x250bp72 / 47 Hours48 / 39 Hours3 / 28 Hours
Small RNA Sequencing1M Reads1x36bp12 / 4 Hours
Library Quality Control 0,5M Reads2x25bp48 / 5,5 Hours